iPSC induction protocol

Induction methodRequiredipsc_induction_protocol.method (string enum) Induction method applied to primary cell culture to induce pluripotent stem cell generation. Should be one of: "lentivirus", "sendai virus", "Gun particle", "piggyBac transposon", "miRNA viral", "adenovirus", "cre-loxP", "plasmid" or "retroviral".

Percent pluripotencyipsc_induction_protocol.percent_pluripotency (number) Percent of iPSCs that passed the pluripotency test. For example "97.2".

Pluripotency testipsc_induction_protocol.pluripotency_test (string) Description of how pluripotency was tested in induced pluripotent stem cells. For example "Teratoma formation", "PluriTest" or "Rt-PCR".

Pluripotent vector removedipsc_induction_protocol.pluripotency_vector_removed (string enum) Whether a viral vector was removed after induction. Should be one of: "yes", "no" or "unknown".

Reprogramming factorsipsc_induction_protocol.reprogramming_factors (string) Reprogramming factors added to primary cell culture to induce pluripotency. For example "POU5F1", "SOX2", "KLF4" or "c-MYC".

iPSC induction protocol>Induction kit (purchased_reagents object) Kit used to induce pluripotent stem cell generation.

Catalog numberipsc_induction_protocol.ipsc_induction_kit.catalog_number (string from purchased_reagents) The catalog number of the kit/reagent. For example "20014279".

Expiry dateipsc_induction_protocol.ipsc_induction_kit.expiry_date (string from purchased_reagents) The date of expiration for the kit/reagent. For example "2018-01-31" or "2018-01".

Titeripsc_induction_protocol.ipsc_induction_kit.kit_titer (string from purchased_reagents) Appropriate titer and volume recommendations found in kit/reagent Certificate of Analysis. For example "3.0x10^7".

Batch/lot numberipsc_induction_protocol.ipsc_induction_kit.lot_number (string from purchased_reagents) The batch or lot number of the kit/reagent. For example "10001A".

Manufactureripsc_induction_protocol.ipsc_induction_kit.manufacturer (string from purchased_reagents) The manufacturer of the kit/reagent. For example "Illumina" or "ThermoFisher Scientific".

Retail nameipsc_induction_protocol.ipsc_induction_kit.retail_name (string from purchased_reagents) The retail name of the kit/reagent. For example "SureCell WTA 3' Library Prep Kit" or "CytoTune iPS 2.0 Sendai Reprogramming Kit".

iPSC induction protocol>Additional reagents (purchased_reagents array) Additional purchased reagents used in this protocol.

Catalog numberipsc_induction_protocol.reagents.catalog_number (string from purchased_reagents) The catalog number of the kit/reagent. For example "20014279".

Expiry dateipsc_induction_protocol.reagents.expiry_date (string from purchased_reagents) The date of expiration for the kit/reagent. For example "2018-01-31" or "2018-01".

Titeripsc_induction_protocol.reagents.kit_titer (string from purchased_reagents) Appropriate titer and volume recommendations found in kit/reagent Certificate of Analysis. For example "3.0x10^7".

Batch/lot numberipsc_induction_protocol.reagents.lot_number (string from purchased_reagents) The batch or lot number of the kit/reagent. For example "10001A".

Manufactureripsc_induction_protocol.reagents.manufacturer (string from purchased_reagents) The manufacturer of the kit/reagent. For example "Illumina" or "ThermoFisher Scientific".

Retail nameipsc_induction_protocol.reagents.retail_name (string from purchased_reagents) The retail name of the kit/reagent. For example "SureCell WTA 3' Library Prep Kit" or "CytoTune iPS 2.0 Sendai Reprogramming Kit".

iPSC induction protocol>Protocol coreRequired (protocol_core object) Core protocol-level information.

Document filenameipsc_induction_protocol.protocol_core.document (string from protocol_core) A filename of a PDF document containing the details of the protocol. For example "my_cool_protocol.pdf".

Protocol descriptionipsc_induction_protocol.protocol_core.protocol_description (string from protocol_core) A general description of the protocol.

Protocol IDRequiredipsc_induction_protocol.protocol_core.protocol_id (string from protocol_core) A unique ID for the protocol.

Protocol nameipsc_induction_protocol.protocol_core.protocol_name (string from protocol_core) A short name for the protocol.

protocols.io DOIipsc_induction_protocol.protocol_core.protocols_io_doi (string from protocol_core) The protocols.io digital object identifier (doi) for the protocol. For example "10.17504/protocols.io.mgjc3un".

Publication DOIipsc_induction_protocol.protocol_core.publication_doi (string from protocol_core) The publication digital object identifier (doi) for the protocol. For example "10.1101/193219".

iPSC induction protocol>provenance (provenance object) Provenance information provided by the system.

Accessionipsc_induction_protocol.provenance.accession (string from provenance) A unique accession for this entity, provided by the broker.

Document IDRequiredipsc_induction_protocol.provenance.document_id (string from provenance) Identifier for document.

Schema major versionipsc_induction_protocol.provenance.schema_major_version (integer from provenance) The major version number of the schema. For example "4" or "10".

Schema minor versionipsc_induction_protocol.provenance.schema_minor_version (integer from provenance) The minor version number of the schema. For example "6" or "15".

Submission dateRequiredipsc_induction_protocol.provenance.submission_date (string from provenance) When project was first submitted to database.

Submitter IDipsc_induction_protocol.provenance.submitter_id (string from provenance) ID of individual who first submitted project.

Update dateipsc_induction_protocol.provenance.update_date (string from provenance) When project was last updated.

Updater IDipsc_induction_protocol.provenance.updater_id (string from provenance) ID of individual who last updated project.