Differentiation protocol

Information about how a cell is differentiated to a desired cell type or organoid.

Properties

Differentiation mediadifferentiation_protocol.media (string) Culture media used to induce a specific differentiation response. For example "RPMI 1640 + B27", "Neurobasal Media" or "StemPro-34 Serum-Free Medium".

Differentiation methodRequireddifferentiation_protocol.method (string) Method applied to cell culture to induce a specific differentiation response. For example "Embryoid Body", "Monolayer" or "Inductive Co-Culture".

Small moleculesdifferentiation_protocol.small_molecules (string) Small molecules added to stem cell medium to induce a specific differentiation response. For example "Retinoic Acid", "CHIR99021 (GSK-inhibitor)", "Activin A" or "BMP4".

Percent target cell yielddifferentiation_protocol.target_cell_yield (number) Percent of target cells obtained after directed differentiation of origin cell. For example "95".

Target pathwaydifferentiation_protocol.target_pathway (string) Targeted pathway for specific differentiation response. For example "Wnt pathway" or "Hedgehog signaling pathway".

Differentiation validation methoddifferentiation_protocol.validation_method (string) Method used to validate origin cell successfully differentiated to target cell. For example "Pancreatic Cell DTZ Detection Assay", "qPCR", "Flow Cytometry" or "Immunocytochemistry Staining".

Validation resultdifferentiation_protocol.validation_result (string) Result confirming successful differentiation to target cell type. For example "CD103 Positive", "Nestin Positive", "HCN4 Positive" or "CD11C Negative".

Catalog numberdifferentiation_protocol.reagents.catalog_number (string from purchased_reagents) The catalog number of the kit/reagent. For example "20014279".

Expiry datedifferentiation_protocol.reagents.expiry_date (string from purchased_reagents) The date of expiration for the kit/reagent. For example "2018-01-31" or "2018-01".

Titerdifferentiation_protocol.reagents.kit_titer (string from purchased_reagents) Appropriate titer and volume recommendations found in kit/reagent Certificate of Analysis. For example "3.0x10^7".

Batch/lot numberdifferentiation_protocol.reagents.lot_number (string from purchased_reagents) The batch or lot number of the kit/reagent. For example "10001A".

Manufacturerdifferentiation_protocol.reagents.manufacturer (string from purchased_reagents) The manufacturer of the kit/reagent. For example "Illumina" or "ThermoFisher Scientific".

Retail namedifferentiation_protocol.reagents.retail_name (string from purchased_reagents) The retail name of the kit/reagent. For example "SureCell WTA 3' Library Prep Kit" or "CytoTune iPS 2.0 Sendai Reprogramming Kit".

Document filenamedifferentiation_protocol.protocol_core.document (string from protocol_core) A filename of a PDF document containing the details of the protocol. For example "my_cool_protocol.pdf".

Protocol descriptiondifferentiation_protocol.protocol_core.protocol_description (string from protocol_core) A general description of the protocol.

Protocol IDRequireddifferentiation_protocol.protocol_core.protocol_id (string from protocol_core) A unique ID for the protocol.

Protocol namedifferentiation_protocol.protocol_core.protocol_name (string from protocol_core) A short name for the protocol.

protocols.io DOIdifferentiation_protocol.protocol_core.protocols_io_doi (string from protocol_core) The protocols.io digital object identifier (doi) for the protocol. For example "10.17504/protocols.io.mgjc3un".

Publication DOIdifferentiation_protocol.protocol_core.publication_doi (string from protocol_core) The publication digital object identifier (doi) for the protocol. For example "10.1101/193219".

Accessiondifferentiation_protocol.provenance.accession (string from provenance) A unique accession for this entity, provided by the broker.

Document IDRequireddifferentiation_protocol.provenance.document_id (string from provenance) Identifier for document.

Schema major versiondifferentiation_protocol.provenance.schema_major_version (integer from provenance) The major version number of the schema. For example "4" or "10".

Schema minor versiondifferentiation_protocol.provenance.schema_minor_version (integer from provenance) The minor version number of the schema. For example "6" or "15".

Submission dateRequireddifferentiation_protocol.provenance.submission_date (string from provenance) When project was first submitted to database.

Submitter IDdifferentiation_protocol.provenance.submitter_id (string from provenance) ID of individual who first submitted project.

Update datedifferentiation_protocol.provenance.update_date (string from provenance) When project was last updated.

Updater IDdifferentiation_protocol.provenance.updater_id (string from provenance) ID of individual who last updated project.