HCA Data Portal

Differentiation protocol

Information about how a cell is differentiated to a desired cell type or organoid.

View JSON source: differentiation_protocoldoneShow required fields only


Differentiation mediadifferentiation_protocol.mediacopy (string) Culture media used to induce a specific differentiation response. For example "RPMI 1640 + B27", "Neurobasal Media" or "StemPro-34 Serum-Free Medium".
Differentiation methodRequireddifferentiation_protocol.methodcopy (string) Method applied to cell culture to induce a specific differentiation response. For example "Embryoid Body", "Monolayer" or "Inductive Co-Culture".
Small moleculesdifferentiation_protocol.small_moleculescopy (string) Small molecules added to stem cell medium to induce a specific differentiation response. For example "Retinoic Acid", "CHIR99021 (GSK-inhibitor)", "Activin A" or "BMP4".
Percent target cell yielddifferentiation_protocol.target_cell_yieldcopy (number) Percent of target cells obtained after directed differentiation of origin cell. For example "95".
Target pathwaydifferentiation_protocol.target_pathwaycopy (string) Targeted pathway for specific differentiation response. For example "Wnt pathway" or "Hedgehog signaling pathway".
Differentiation validation methoddifferentiation_protocol.validation_methodcopy (string) Method used to validate origin cell successfully differentiated to target cell. For example "Pancreatic Cell DTZ Detection Assay", "qPCR", "Flow Cytometry" or "Immunocytochemistry Staining".
Validation resultdifferentiation_protocol.validation_resultcopy (string) Result confirming successful differentiation to target cell type. For example "CD103 Positive", "Nestin Positive", "HCN4 Positive" or "CD11C Negative".
Differentiation protocol>Differentiation reagents (purchased_reagents array) A list of purchased reagents used in the differentiation protocol.
Catalog numberdifferentiation_protocol.reagents.catalog_numbercopy (string from purchased_reagents) The catalog number of the kit/reagent. For example "20014279".
Expiry datedifferentiation_protocol.reagents.expiry_datecopy (string from purchased_reagents) The date of expiration for the kit/reagent. For example "2018-01-31" or "2018-01".
Titerdifferentiation_protocol.reagents.kit_titercopy (string from purchased_reagents) Appropriate titer and volume recommendations found in kit/reagent Certificate of Analysis. For example "3.0x10^7".
Batch/lot numberdifferentiation_protocol.reagents.lot_numbercopy (string from purchased_reagents) The batch or lot number of the kit/reagent. For example "10001A".
Manufacturerdifferentiation_protocol.reagents.manufacturercopy (string from purchased_reagents) The manufacturer of the kit/reagent. For example "Illumina" or "ThermoFisher Scientific".
Retail namedifferentiation_protocol.reagents.retail_namecopy (string from purchased_reagents) The retail name of the kit/reagent. For example "SureCell WTA 3' Library Prep Kit" or "CytoTune iPS 2.0 Sendai Reprogramming Kit".
Differentiation protocol>Protocol coreRequired (protocol_core object) Core protocol-level information.
Document filenamedifferentiation_protocol.protocol_core.documentcopy (string from protocol_core) A filename of a PDF document containing the details of the protocol. For example "my_cool_protocol.pdf".
Protocol descriptiondifferentiation_protocol.protocol_core.protocol_descriptioncopy (string from protocol_core) A general description of the protocol.
Protocol IDRequireddifferentiation_protocol.protocol_core.protocol_idcopy (string from protocol_core) A unique ID for the protocol.
Protocol namedifferentiation_protocol.protocol_core.protocol_namecopy (string from protocol_core) A short name for the protocol.
protocols.io DOIdifferentiation_protocol.protocol_core.protocols_io_doicopy (string from protocol_core) The protocols.io digital object identifier (doi) for the protocol. For example "10.17504/protocols.io.mgjc3un".
Publication DOIdifferentiation_protocol.protocol_core.publication_doicopy (string from protocol_core) The publication digital object identifier (doi) for the protocol. For example "10.1101/193219".
Differentiation protocol>provenance (provenance object) Provenance information provided by the system.
Accessiondifferentiation_protocol.provenance.accessioncopy (string from provenance) A unique accession for this entity, provided by the broker.
Document IDRequireddifferentiation_protocol.provenance.document_idcopy (string from provenance) Identifier for document.
Schema major versiondifferentiation_protocol.provenance.schema_major_versioncopy (integer from provenance) The major version number of the schema. For example "4" or "10".
Schema minor versiondifferentiation_protocol.provenance.schema_minor_versioncopy (integer from provenance) The minor version number of the schema. For example "6" or "15".
Submission dateRequireddifferentiation_protocol.provenance.submission_datecopy (string from provenance) When project was first submitted to database.
Submitter IDdifferentiation_protocol.provenance.submitter_idcopy (string from provenance) ID of individual who first submitted project.
Update datedifferentiation_protocol.provenance.update_datecopy (string from provenance) When project was last updated.
Updater IDdifferentiation_protocol.provenance.updater_idcopy (string from provenance) ID of individual who last updated project.