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Metadata

iPSC induction protocol

Information about how a biomaterial is treated to become an induced pluripotent stem cell.

View JSON source: ipsc_induction_protocoldoneShow required fields only

Properties

Induction methodRequiredipsc_induction_protocol.methodfile_copy (string enum) Induction method applied to primary cell culture to induce pluripotent stem cell generation. Should be one of: "lentivirus", "sendai virus", "Gun particle", "piggyBac transposon", "miRNA viral", "adenovirus", "cre-loxP", "plasmid" or "retroviral".
Percent pluripotencyipsc_induction_protocol.percent_pluripotencyfile_copy (number) Percent of iPSCs that passed the pluripotency test. For example "97.2".
Pluripotency testipsc_induction_protocol.pluripotency_testfile_copy (string) Description of how pluripotency was tested in induced pluripotent stem cells. For example "Teratoma formation", "PluriTest" or "Rt-PCR".
Pluripotent vector removedipsc_induction_protocol.pluripotency_vector_removedfile_copy (string enum) Whether a viral vector was removed after induction. Should be one of: "yes", "no" or "unknown".
Reprogramming factorsipsc_induction_protocol.reprogramming_factorsfile_copy (string) Reprogramming factors added to primary cell culture to induce pluripotency. For example "POU5F1", "SOX2", "KLF4" or "c-MYC".
iPSC induction protocol>Induction kit (purchased_reagents) Kit used to induce pluripotent stem cell generation.
Catalog numberipsc_induction_protocol.ipsc_induction_kit.catalog_numberfile_copy (string from purchased_reagents) The catalog number of the kit/reagent. For example "20014279".
Expiry dateipsc_induction_protocol.ipsc_induction_kit.expiry_datefile_copy (string from purchased_reagents) The date of expiration for the kit/reagent. For example "2018-01-31" or "2018-01".
Titeripsc_induction_protocol.ipsc_induction_kit.kit_titerfile_copy (string from purchased_reagents) Appropriate titer and volume recommendations found in kit/reagent Certificate of Analysis. For example "3.0x10^7".
Batch/lot numberipsc_induction_protocol.ipsc_induction_kit.lot_numberfile_copy (string from purchased_reagents) The batch or lot number of the kit/reagent. For example "10001A".
Manufactureripsc_induction_protocol.ipsc_induction_kit.manufacturerfile_copy (string from purchased_reagents) The manufacturer of the kit/reagent. For example "Illumina" or "ThermoFisher Scientific".
Retail nameipsc_induction_protocol.ipsc_induction_kit.retail_namefile_copy (string from purchased_reagents) The retail name of the kit/reagent. For example "SureCell WTA 3' Library Prep Kit" or "CytoTune iPS 2.0 Sendai Reprogramming Kit".
iPSC induction protocol>Additional reagents (purchased_reagents) Additional purchased reagents used in this protocol.
Catalog numberipsc_induction_protocol.reagents.catalog_numberfile_copy (string from purchased_reagents) The catalog number of the kit/reagent. For example "20014279".
Expiry dateipsc_induction_protocol.reagents.expiry_datefile_copy (string from purchased_reagents) The date of expiration for the kit/reagent. For example "2018-01-31" or "2018-01".
Titeripsc_induction_protocol.reagents.kit_titerfile_copy (string from purchased_reagents) Appropriate titer and volume recommendations found in kit/reagent Certificate of Analysis. For example "3.0x10^7".
Batch/lot numberipsc_induction_protocol.reagents.lot_numberfile_copy (string from purchased_reagents) The batch or lot number of the kit/reagent. For example "10001A".
Manufactureripsc_induction_protocol.reagents.manufacturerfile_copy (string from purchased_reagents) The manufacturer of the kit/reagent. For example "Illumina" or "ThermoFisher Scientific".
Retail nameipsc_induction_protocol.reagents.retail_namefile_copy (string from purchased_reagents) The retail name of the kit/reagent. For example "SureCell WTA 3' Library Prep Kit" or "CytoTune iPS 2.0 Sendai Reprogramming Kit".
iPSC induction protocol>Protocol coreRequired (protocol_core) Core protocol-level information.
Document filenameipsc_induction_protocol.protocol_core.documentfile_copy (string from protocol_core) A filename of a PDF document containing the details of the protocol. For example "my_cool_protocol.pdf".
Protocol descriptionipsc_induction_protocol.protocol_core.protocol_descriptionfile_copy (string from protocol_core) A general description of the protocol.
Protocol IDRequiredipsc_induction_protocol.protocol_core.protocol_idfile_copy (string from protocol_core) A unique ID for the protocol.
Protocol nameipsc_induction_protocol.protocol_core.protocol_namefile_copy (string from protocol_core) A short name for the protocol.
protocols.io DOIipsc_induction_protocol.protocol_core.protocols_io_doifile_copy (string from protocol_core) The protocols.io digital object identifier (doi) for the protocol. For example "10.17504/protocols.io.mgjc3un".
Publication DOIipsc_induction_protocol.protocol_core.publication_doifile_copy (string from protocol_core) The publication digital object identifier (doi) for the protocol. For example "10.1101/193219".
iPSC induction protocol>provenance (provenance) Provenance information provided by the system.
Accessionipsc_induction_protocol.provenance.accessionfile_copy (string from provenance) A unique accession for this entity, provided by the broker.
Document IDRequiredipsc_induction_protocol.provenance.document_idfile_copy (string from provenance) Identifier for document.
Schema major versionipsc_induction_protocol.provenance.schema_major_versionfile_copy (integer from provenance) The major version number of the schema. For example "4" or "10".
Schema minor versionipsc_induction_protocol.provenance.schema_minor_versionfile_copy (integer from provenance) The minor version number of the schema. For example "6" or "15".
Submission dateRequiredipsc_induction_protocol.provenance.submission_datefile_copy (string from provenance) When project was first submitted to database.
Submitter IDipsc_induction_protocol.provenance.submitter_idfile_copy (string from provenance) ID of individual who first submitted project.
Update dateipsc_induction_protocol.provenance.update_datefile_copy (string from provenance) When project was last updated.
Updater IDipsc_induction_protocol.provenance.updater_idfile_copy (string from provenance) ID of individual who last updated project.