HCA Data Portal
Gut Network

Gut Network

The HCA datasets below focus on the network's tissues and are potential source datasets for integrated atlases.

Project TitleSpeciesMethodAnatomical EntityDisease (Donor)Cell Count Est.
A cell atlas of human thymic development defines T cell repertoire formation
2 species
4 library construction methods
2 anatomical entities
normal

250,000

A human cell atlas of fetal gene expression.

Homo sapiens

sci-RNA-seq3

14 anatomical entities
normal

4,062,965

A proximal-to-distal survey of healthy adult human small intestine and colon epithelium by single-cell transcriptomics

Homo sapiens

10x 3' v3

2 anatomical entities
normal

12,590

A single-cell atlas of chromatin accessibility in the human genome

Homo sapiens

sci-RNA-seq

18 anatomical entities
normal

500,000

An organoid and multi-organ developmental cell atlas reveals multilineage fate specification in the human intestine

Homo sapiens

2 library construction methods
5 anatomical entities

Unspecified

Unspecified

Assessing Cellular and Transcriptional diversity of IIeal Mucosa amongst Treatment Naïve and Treated Crohn’s disease

Homo sapiens

10x 5' v1

ileum

normal

Crohn's disease

139,906

Benchmarking Single-Cell RNA Sequencing Protocols for Cell Atlas Projects
3 species
12 library construction methods
4 anatomical entities
normal

50,556

Cells of the human intestinal tract mapped across space and time

Homo sapiens

2 library construction methods
4 anatomical entities
normal

type 2 diabetes mellitus

428,000

Colonic single epithelial cell census reveals goblet cell drivers of barrier breakdown in inflammatory bowel disease.

Homo sapiens

10X 3' v2 sequencing

digestive system

normal

ulcerative colitis (disease)

11,175

Construction of A Human Cell Landscape by Single-cell mRNA-seq

Homo sapiens

Microwell-Seq

37 anatomical entities
normal
19 diseases

702,968

Cross-tissue immune cell analysis reveals tissue-specific features in humans

Homo sapiens

6 library construction methods
10 anatomical entities
normal

357,211

Differential pre-malignant programs and microenvironment chart distinct paths to malignancy in human colorectal polyps

Homo sapiens

TruDrop

colon

polyp of colon

68,301

Differentiation of Human Intestinal Organoids with Endogenous Vascular Endothelial Cells

Homo sapiens

2 library construction methods
3 anatomical entities

Unspecified

Unspecified

Distinct microbial and immune niches of the human colon

Homo sapiens

5 library construction methods
2 anatomical entities
normal

1,522

Genome-Wide DNA Hypermethylation in the Wound-Edge of Chronic Wound Patients Opposes Closure by Impairing Epithelial to Mesenchymal Transition

Homo sapiens

2 library construction methods
3 anatomical entities
normal

type 2 diabetes mellitus

36,487

Heterogeneity and clonal relationships of adaptive immune cells in ulcerative colitis revealed by single-cell analyses

Homo sapiens

10x immune profiling

3 anatomical entities
normal

inflammatory bowel disease

121,368

Identification of a unique subset of tissue-resident memory CD4+ T cells in Crohn’s disease

Homo sapiens

2 library construction methods
2 anatomical entities
3 diseases

25,171

Intra- and Inter-cellular Rewiring of the Human Colon during Ulcerative Colitis.

Homo sapiens

2 library construction methods
7 anatomical entities
normal

ulcerative colitis

366,650

Lineage-dependent gene expression programs influence the immune landscape of colorectal cancer.

Homo sapiens

10x 3' v2

large intestine

colorectal cancer

109,512

Mapping Development of the Human Intestinal Niche at Single-Cell Resolution

Homo sapiens

2 library construction methods

small intestine

Unspecified

Unspecified

Mapping the developing human immune system across organs
2 species
5 library construction methods
10 anatomical entities
normal

922,332

Mucosal Profiling of Pediatric-Onset Colitis and IBD Reveals Common Pathogenics and Therapeutic Pathways

Homo sapiens

3 library construction methods

colon

normal
2 diseases

73,165

Reconstructing the human first trimester fetal-maternal interface using single cell transcriptomics

Homo sapiens

2 library construction methods
3 anatomical entities
normal

orofaciodigital syndrome VIII

70,000

Refining Colorectal Cancer Classification and Clinical Stratification Through a Single-Cell Atlas

Homo sapiens

10x 5' transcription profiling

colon

colorectal cancer

49,589

SARS-CoV-2 receptor ACE2 is an interferon-stimulated gene in human airway epithelial cells and is detected in specific cell subsets across tissues.
2 species
4 library construction methods
4 anatomical entities
normal

33,844

Single cell analysis of endometriosis reveals a coordinated transcriptional program driving immunotolerance and angiogenesis across eutopic and ectopic tissues

Homo sapiens

2 library construction methods
3 anatomical entities
normal

endometriosis

122,000

Single cell transcriptomic analysis of the immune cell compartment in the human small intestine and in Celiac disease

Homo sapiens

SORT-seq

small intestine

normal
3 diseases

4,000

Single-Cell Atlas of Lineage States, Tumor Microenvironment, and Subtype-Specific Expression Programs in Gastric Cancer

Homo sapiens

10x TCR enrichment

2 anatomical entities

gastric adenocarcinoma

200,000

Single-Cell Transcriptome and Downstream Analyses of Human Stomach Reveal the Cell Diversity and Developmental Features of Gastric and Metaplastic Mucosae

Homo sapiens

2 library construction methods

stomach

normal

137,610

Single-cell analyses of Crohn's disease tissues reveal intestinal intraepithelial T cells heterogeneity and altered subset distributions

Homo sapiens

10x 3' v3

2 anatomical entities
4 diseases

29,247

Single-cell analysis of Crohn’s disease lesions identifies a pathogenic cellular module associated with resistance to anti-TNF therapy

Homo sapiens

10x 3' transcription profiling

2 anatomical entities

Crohn ileitis

Unspecified

Single-cell analysis of gastric pre-cancerous and cancer lesions reveals cell lineage diversity and intratumoral heterogeneity

Homo sapiens

10x 3' v2

stomach

3 diseases

13,022

Single-cell atlas of diverse immune populations in the advanced biliary tract cancer microenvironment

Homo sapiens

2 library construction methods
5 anatomical entities
3 diseases

45,851

Single-cell deciphering of human fetal innate lymphoid cell development

Homo sapiens

10x 3' v3

6 anatomical entities
normal

Unspecified

Single-cell transcriptome profiling of an adult human cell atlas of 15 major organs

Homo sapiens

2 library construction methods
15 anatomical entities
normal

84,363

Single-cell transcriptomic analyses provide insights into the developmental origins of neuroblastoma.

Homo sapiens

4 library construction methods
8 anatomical entities
normal

neuroblastoma

220,527

Single-nucleus cross-tissue molecular reference maps toward understanding disease gene function.

Homo sapiens

10x 3' v2

7 anatomical entities
normal

209,126

Spatial and single-cell transcriptional profiling identifies functionally distinct human dermal fibroblast subpopulations

Homo sapiens

Smart-seq2

abdomen

normal

184

Spatially distinct reprogramming of the tumor microenvironment based on tumor invasion in diffuse-type gastric cancers

Homo sapiens

10x 3' v2

stomach

gastric cancer

30,365

Spatially organized multicellular immune hubs in human colorectal cancer

Homo sapiens

2 library construction methods

colon

colorectal adenocarcinoma

371,223

Spatiotemporal Analysis of Human Intestinal Development at Single Cell Resolution - scRNA-Seq

Homo sapiens

2 library construction methods
3 anatomical entities
normal
2 diseases

98,866

Structural Remodeling of the Human Colonic Mesenchyme in Inflammatory Bowel Disease
2 species
2 library construction methods

colon

normal
2 diseases

16,893

Tabula Muris: Transcriptomic characterization of 20 organs and tissues from Mus musculus at single cell resolution

Mus musculus

Smart-seq2

18 anatomical entities
normal

100,605

The Human and Mouse Enteric Nervous System at Single-Cell Resolution.
2 species
2 library construction methods
2 anatomical entities
normal

1,600,000

The Immune Atlas of Human Deciduas With Unexplained Recurrent Pregnancy Loss

Homo sapiens

10x 3' v3 sequencing

decidua

normal

14,000

The Tabula Sapiens: A multiple-organ, single-cell transcriptomic atlas of humans

Homo sapiens

3 library construction methods
24 anatomical entities
normal
10 diseases

481,120

The emergent landscape of the mouse gut endoderm at single-cell resolution

Mus musculus

10x 3' v2

3 anatomical entities
normal

112,201

The immune cell atlas of human neuroblastoma

Homo sapiens

10x 3' v2

7 anatomical entities

neuroblastoma

46,261

The landscape of immune dysregulation in Crohn's disease revealed through single-cell transcriptomic profiling in the ileum and colon

Homo sapiens

3 library construction methods
3 anatomical entities
2 diseases

720,633

The myogenesis program drives clonal selection and drug resistance in rhabdomyosarcoma

Homo sapiens

5 library construction methods
12 anatomical entities
2 diseases

764,251

Ulcerative colitis is characterized by a plasmablast-skewed humoral response associated with disease activity

Homo sapiens

10x 3' v2

colon

normal

inflammatory bowel disease

40,327

scRNA-seq assessment of the human lung, spleen, and esophagus tissue stability after cold preservation

Homo sapiens

3 library construction methods
3 anatomical entities
normal

199,845

scRNA-sequencing reveals new enteric nervous system roles for GDNF, NRTN, and TBX3
2 species

10x 3' v2

2 anatomical entities
normal
11 diseases

11,944