HCA Data Explorer

Single-cell reconstruction of follicular remodeling in the human adult ovary

Updated September 10, 2021

The ovary is perhaps the most dynamic organ in the human body, only rivaled by the uterus. The molecular mechanisms that regulate follicular growth and regression, ensuring ovarian tissue homeostasis, remain elusive. We have performed single-cell RNA-sequencing using human adult ovaries to provide a map of the molecular signature of growing and regressing follicular populations. We have identified different types of granulosa and theca cells and detected local production of components of the complement system by (atretic) theca cells and stromal cells. We also have detected a mixture of adaptive and innate immune cells, as well as several types of endothelial and smooth muscle cells to aid the remodeling process. Our results highlight the relevance of mapping whole adult organs at the single-cell level and reflect ongoing efforts to map the human body. The association between complement system and follicular remodeling may provide key insights in reproductive biology and (in)fertility.

Ioannis MoustakasLeiden University Medical Centerioannis.moustakas1@gmail.com
Ioannis Moustakas (Experimental Scientist)1
1Leiden University Medical Center
Parisa Nejad
Kamron Mojabe
Pranav Muthuraman

To reference this project, please use the following link:

https://explore.data.humancellatlas.org/projects/faeedcb0-e046-4be7-b1ad-80a3eeabb066
None
GEO Series Accessions:

For information regarding data sharing and data use, please see our Data Access Policy.

Analysis Portals

CZ CELLxGENECZ CELLxGENE
UCSC Cell BrowserUCSC Cell Browser

Project Label

Human5Ovary10xChuva

Species

Homo sapiens

Sample Type

specimens

Anatomical Entity

ovary

Organ Part

2 organ parts

Selected Cell Types

2 cell types

Disease Status (Specimen)

normal

Disease Status (Donor)

cancer

Development Stage

human adult stage

Library Construction Method

10x 3' V2 sequencing

Nucleic Acid Source

single cell

Paired End

false

Analysis Protocol

analysis_protocol_1

File Format

2 file formats

Cell Count Estimate

Unspecified

Donor Count

5
fastq.gz228 file(s)h531 file(s)