HCA Data Explorer

Single-cell atlas of early human brain development highlights heterogeneity of human neuroepithelial cells and early radial glia.

Updated August 30, 2022

The human cortex comprises diverse cell types that emerge from an initially uniform neuroepithelium that gives rise to radial glia, the neural stem cells of the cortex. To characterize the earliest stages of human brain development, we performed single-cell RNA-sequencing across regions of the developing human brain, including the telencephalon, diencephalon, midbrain, hindbrain and cerebellum. We identify nine progenitor populations physically proximal to the telencephalon, suggesting more heterogeneity than previously described, including a highly prevalent mesenchymal-like population that disappears once neurogenesis begins. Comparison of human and mouse progenitor populations at corresponding stages identifies two progenitor clusters that are enriched in the early stages of human cortical development. We also find that organoid systems display low fidelity to neuroepithelial and early radial glia cell types, but improve as neurogenesis progresses. Overall, we provide a comprehensive molecular and spatial atlas of early stages of human brain and cortical development.

Aparna BhaduriUniversity of California, San Francisco (UCSF)Aparna.Bhaduri@ucsf.edu
Arnold R KriegsteinUniversity of California, San Francisco (UCSF)Arnold.Kriegstein@ucsf.edu
Ugomma C Eze1
Aparna Bhaduri1
Maximilian Haeussler2
Tomasz J Nowakowski1
Arnold R Kriegstein (Principal Investigator)1
1University of California, San Francisco (UCSF)
2University of California, Santa Cruz
Enrique Sapena Ventura

To reference this project, please use the following link:

https://explore.data.humancellatlas.org/projects/ef1d9888-fa86-47a4-bb72-0ab0f20f7004

Supplementary links are provided by contributors and represent items such as additional data which can’t be hosted here; code that was used to analyze this data; or tools and visualizations associated with this specific dataset.

1.https://assets.nemoarchive.org/dat-0rsydy72.https://cells-test.gi.ucsc.edu/?ds=early-brain
None

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Analysis Portals

UCSC Cell BrowserUCSC Cell Browser

Project Label

dcp1219560702433721

Species

Homo sapiens

Sample Type

specimens

Anatomical Entity

2 anatomical entities

Organ Part

17 organ parts

Selected Cell Types

Unspecified

Disease Status (Specimen)

normal

Disease Status (Donor)

normal

Development Stage

7 development stages

Library Construction Method

10x 3' v2

Nucleic Acid Source

single cell

Paired End

false

Analysis Protocol

annotation_generation, matrix_generation

File Format

4 file formats

Cell Count Estimate

289.0k

Donor Count

10
tar.gz63 file(s)tsv1 file(s)tsv.gz2 file(s)xlsx1 file(s)