HCA Data Explorer

Single-Cell Transcriptomics Reveals a Population of Dormant Neural Stem Cells that Become Activated upon Brain Injury.

Updated August 30, 2022

Heterogeneous pools of adult neural stem cells (NSCs) contribute to brain maintenance and regeneration after injury. The balance of NSC activation and quiescence, as well as the induction of lineage-specific transcription factors, may contribute to diversity of neuronal and glial fates. To identify molecular hallmarks governing these characteristics, we performed single-cell sequencing of an unbiased pool of adult subventricular zone NSCs. This analysis identified a discrete, dormant NSC subpopulation that already expresses distinct combinations of lineage-specific transcription factors during homeostasis. Dormant NSCs enter a primed-quiescent state before activation, which is accompanied by downregulation of glycolytic metabolism, Notch, and BMP signaling and a concomitant upregulation of lineage-specific transcription factors and protein synthesis. In response to brain ischemia, interferon gamma signaling induces dormant NSC subpopulations to enter the primed-quiescent state. This study unveils general principles underlying NSC activation and lineage priming and opens potential avenues for regenerative medicine in the brain.

Ana Martin-VillalbaGerman Cancer Research Centera.martin-villalba@dkfz.de
Enric Llorens-Bobadilla1
Sheng Zhao1
Avni Baser1
Gonzalo Saiz-Castro1
Klara Zwadlo1
Ana Martin-Villalba1
1German Cancer Research Center
Ray Stefancsik

To reference this project, please use the following link:

https://explore.data.humancellatlas.org/projects/e8808cc8-4ca0-4096-80f2-bba73600cba6
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Analysis Portals

None

Project Label

GSE67833_neural_stem_cells

Species

Mus musculus

Sample Type

specimens

Anatomical Entity

3 anatomical entities

Organ Part

Unspecified

Selected Cell Types

Unspecified

Disease Status (Specimen)

Unspecified

Disease Status (Donor)

Unspecified

Development Stage

adult

Library Construction Method

Smart-seq2

Nucleic Acid Source

single cell

Paired End

true

Analysis Protocol

MultiSampleSmartSeq2_v2.2.6, SmartSeq2SingleSample_v5.1.5

File Format

6 file formats

Cell Count Estimate

272

Donor Count

6
bai897 file(s)bam897 file(s)csv.gz2 file(s)fastq.gz1,794 file(s)loom3 file(s)xlsx1 file(s)