Kidney micro-organoids in suspension culture as a scalable source of human pluripotent stem cell-derived kidney cells.
Updated November 29, 2022Kidney organoids have potential uses in disease modelling, drug screening and regenerative medicine. However, novel cost-effective techniques are needed to enable scaled-up production of kidney cell types in vitro. The authors describe here a modified suspension culture method for the generation of kidney micro-organoids from human pluripotent stem cells. Optimisation of differentiation conditions allowed the formation of micro-organoids, each containing six to ten nephrons that were surrounded by endothelial and stromal populations. Single cell transcriptional profiling confirmed the presence and transcriptional equivalence of all anticipated renal cell types consistent with a previous organoid culture method.
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Analysis Portals
NoneProject Label
GSE117211_KidneyMicroOrganoidsSpecies
Sample Type
Anatomical Entity
Organ Part
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Model Organ
Disease Status (Specimen)
Disease Status (Donor)
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Nucleic Acid Source
Paired End
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analysis_protocolFile Format
Cell Count Estimate
4.0kDonor Count
2