HCA Data Explorer

Single-Cell RNA Sequencing Reveals Human iPS Cell-derived Alveolar Type 1 Cells in Alveolar Organoids

Updated January 23, 2023

We induced differentiation from human induced pluripotent stem cell (hiPSC)-derived alveolar type2 (AT2) cells into hiPSC-derived 2D alveoalr type 1 (2D-iAT1) cells. Single cell RNA sequencing revealed that 2D-iAT1 cells were composed of three clusters. Overall design: After isolation of hiPSC-derived AT2 cells, hiPSC-dervied AT2 cells were cultured in two-demensional condition for 7 days, and then single cell libraries were generated using Fluidigm C1 system. Single "2D_EpCAM+" cells were captured and separated on the each chamber of medium-sized (17-25 µm) C1 integrated fluidic circuit (IFC) with the C1 system (Fluidgm) Then, first-strand cDNA was synthesized and amplified on the independent chamber using SMART-Seq v4 Ultra Low Input RNA Kit for Sequencing.

Shimpei GotohKyoto Universitykonai@kuhp.kyoto-u.ac.jp
Shimpei Gotoh (Experimental Scientist)1
1Kyoto University
Rachel Schwartz

To reference this project, please use the following link:

https://explore.data.humancellatlas.org/projects/dbcd4b1d-31bd-4eb5-94e1-50e8706fa192
None
INSDC Project Accessions:GEO Series Accessions:INSDC Study Accessions:

For information regarding data sharing and data use, please see our Data Access Policy.

Analysis Portals

None

Project Label

HumanIpscDerivedAlveolarOrganoids

Species

Homo sapiens

Sample Type

organoids

Anatomical Entity

dermis

Organ Part

Unspecified

Selected Cell Types

type I pneumocyte

Model Organ

lung

Disease Status (Specimen)

normal

Disease Status (Donor)

normal

Development Stage

human adult stage

Library Construction Method

Smart-seq

Nucleic Acid Source

single cell

Paired End

false

Analysis Protocol

analysis_protocol_1

File Format

3 file formats

Cell Count Estimate

83

Donor Count

1
fastq.gz83 file(s)txt.gz1 file(s)xlsx1 file(s)