HCA Data Explorer

Single-cell sequencing unveils the heterogeneity of nonimmune cells in chronic apical periodontitis

Updated January 26, 2023

Chronic apical periodontitis (CAP) is a unique dynamic interaction between microbial invasions and host defense mechanisms, resulting in bone absorption, infiltration of immune cells and sporadic periapical granuloma. In this study, we constituted a single-cell atlas for 26,737 high-quality cells from hyperplastic periapical tissue using single-cell RNA sequencing. Identifying cell types and signatures at the single-cell level might generate novel insights into the clinical pathogenesis of CAP. A histological analysis to verify the gene signatures of nonimmune cells was combined with immunohistochemistry staining. We then discovered the diversity and heterogeneity of nonimmune cells in regional CAP lesions. The temporal profiling of genomic alterations from common CAP to typical periapical granuloma provided predictions for key transcription factors and biological processes. Our study also inferred that the marked shift of inflammatory cytokines, chemokines, proteases and growth factors enables the initiation of polymorphic cell differentiation, lymphangiogenesis and angiogenesis during CAP. Overall design: Three samples from extracted residual molars with chronic apical periodontitis

Xinwei LinDepartment of Operative Dentistry and Endodontics, Hospital of Stomatology, Sun Yat-sen University, Guangzhou, China.strawberrylinwei@icloud.com

Single-Cell Sequencing Unveils the Heterogeneity of Nonimmune Cells in Chronic Apical Periodontitis.

Xinwei Lin (Principal Investigator)1
1Department of Operative Dentistry and Endodontics, Hospital of Stomatology, Sun Yat-sen University, Guangzhou, China.
Rachel Schwartz

To reference this project, please use the following link:

https://explore.data.humancellatlas.org/projects/d138a114-7df5-4f7d-9ff1-f79dfd2d428f
None
GEO Series Accessions:INSDC Project Accessions:INSDC Study Accessions:

For information regarding data sharing and data use, please see our Data Access Policy.

Analysis Portals

None

Project Label

chronicApicalPeriodontitis

Species

Homo sapiens

Sample Type

specimens

Anatomical Entity

molar tooth

Organ Part

2 organ parts

Selected Cell Types

Unspecified

Disease Status (Specimen)

2 disease statuses

Disease Status (Donor)

chronic apical periodontitis

Development Stage

human adult stage

Library Construction Method

10x 3' v2

Nucleic Acid Source

single cell

Paired End

false

Analysis Protocol

analysis_protocol_1

File Format

3 file formats

Cell Count Estimate

26.7k

Donor Count

3
fastq.gz24 file(s)tar1 file(s)xlsx1 file(s)