HCA Data Explorer

Colonic single epithelial cell census reveals goblet cell drivers of barrier breakdown in inflammatory bowel disease.

Updated July 19, 2023

Three single cell RNA-sequencing (10x, Chromium) experiments were performed on healthy and inflamed human colonic samples, including from patients with Ulcerative Colitis. The authors characterised the composition of the intestinal epithelial cells in these samples.

Alison SimmonsMRC Weatherall Institute of Molecular Medicine (WIMM)alison.simmons@imm.ox.ac.uk
Kaushal Parikh1
Agne Antanaviciute1
David Fawkner-Corbett1
Marta Jagielowicz1
Anna Aulicino1
Christoffer Lagerholm1
Simon Davis2
James Kinchen1
Hannah H Chen1
Nasullah Khalid Alham3
Neil Ashley4
Errin Johnson5
Philip Hublitz4
Leyuan Bao1
Joanna Lukomska1
Rajinder Singh Andev1
Elisabet Björklund1
Benedikt M Kessler2
Roman Fischer2
Robert Goldin6
Hashem Koohy4
Alison Simmons1
1MRC Weatherall Institute of Molecular Medicine (WIMM)
2Target Discovery Institute
3John Radcliffe Hospital, University of Oxford
4MRC Weatherall Institute of Molecular Medicine
5Sir William Dunn School of Pathology, University of Oxford
6St Mary's Hospital, Imperial College
Ami Day

To reference this project, please use the following link:

https://explore.data.humancellatlas.org/projects/c893cb57-5c9f-4f26-9312-21b85be84313

Supplementary links are provided by contributors and represent items such as additional data which can’t be hosted here; code that was used to analyze this data; or tools and visualizations associated with this specific dataset.

1.http://dx.doi.org/10.6019/PXD0116552.https://github.com/agneantanaviciute/colonicepithelium
INSDC Project Accessions:GEO Series Accessions:INSDC Study Accessions:

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Analysis Portals

None

Project Label

EpithelialDiversityHealthInflammation

Species

Homo sapiens

Sample Type

specimens

Anatomical Entity

digestive system

Organ Part

colon

Selected Cell Types

epithelial cell

Disease Status (Specimen)

2 disease statuses

Disease Status (Donor)

2 disease statuses

Development Stage

human adult stage

Library Construction Method

10X 3' v2 sequencing

Nucleic Acid Source

single cell

Paired End

false

Analysis Protocol

4.2.3, optimus_post_processing_v1.0.0

File Format

5 file formats

Cell Count Estimate

11.2k

Donor Count

6
bam9 file(s)fastq.gz135 file(s)loom10 file(s)txt.gz1 file(s)xlsx1 file(s)