Lineage recording in human cerebral organoids.
Updated August 30, 2022Induced pluripotent stem cell (iPSC)-derived organoids provide models to study human organ development. Single-cell transcriptomics enable highly resolved descriptions of cell states within these systems; however, approaches are needed to directly measure lineage relationships. Here we establish iTracer, a lineage recorder that combines reporter barcodes with inducible CRISPR–Cas9 scarring and is compatible with single-cell and spatial transcriptomics. We apply iTracer to explore clonality and lineage dynamics during cerebral organoid development and identify a time window of fate restriction as well as variation in neurogenic dynamics between progenitor neuron families.
Lineage recording in human cerebral organoids. (Official HCA Publication)
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Analysis Portals
NoneProject Label
LineageRecordingCerebralOrganoidsSpecies
Sample Type
Anatomical Entity
Organ Part
Selected Cell Types
Model Organ
Disease Status (Specimen)
Disease Status (Donor)
Development Stage
Library Construction Method
Nucleic Acid Source
Paired End
false, trueAnalysis Protocol
analysis_protocol_10XVisium, analysis_protocol_barcode_and_scar, analysis_protocol_scRNA-seqFile Format
Cell Count Estimate
103.5kDonor Count
1