Robust temporal map of human in vitro myelopoiesis using single-cell genomics.
Updated October 1, 2022Myeloid cells are central to homeostasis and immunity. Characterising in vitro myelopoiesis protocols is imperative for their use in research, immunotherapies, and understanding human myelopoiesis. The authors generated a >470K cells molecular map of human induced pluripotent stem cells (iPSC) differentiation into macrophages. Integration with in vivo single-cell atlases showed in vitro differentiation recapitulates features of yolk sac hematopoiesis, before definitive hematopoietic stem cells (HSC) emerge. The diversity of myeloid cells generated, including mast cells and monocytes, suggests that HSC-independent hematopoiesis can produce multiple myeloid lineages.
Robust temporal map of human in vitro myelopoiesis using single-cell genomics. (Official HCA Publication)
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Analysis Portals
NoneProject Label
TemporalMapHumanMyelopoiesisSpecies
Sample Type
Anatomical Entity
Organ Part
Selected Cell Types
Model Organ
Disease Status (Specimen)
Disease Status (Donor)
Development Stage
Library Construction Method
Nucleic Acid Source
Paired End
falseAnalysis Protocol
analysis_protocol_scRNA-SeqFile Format
Cell Count Estimate
470.0kDonor Count
6