HCA Data Explorer

Single cell RNA-seq of human pancreatic endocrine cells from Juvenile, adult control and type 2 diabetic donors.

Updated January 19, 2022

We successfully sequenced and annotated more than 400 cells from child, adult control, type 1 diabetes and type 2 diabetes donors. We detect donor-type specific transcript variation. We also report that cells from child donors have less defined gene signature. Cells from type 2 diabetes donors resemble juvenile cells in gene expression.

Schug JonathanUniversity of Pennsylvaniajschug@upenn.edu
Schug Jonathan (Principal Investigator)1
1University of Pennsylvania
Parisa Nejad
Beagan Nguy
Kamron Mojabe

To reference this project, please use the following link:

https://explore.data.humancellatlas.org/projects/99101928-d9b1-4aaf-b759-e97958ac7403

Supplementary links are provided by contributors and represent items such as additional data which can’t be hosted here; code that was used to analyze this data; or tools and visualizations associated with this specific dataset.

1.ftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE83nnn/GSE83139/suppl/GSE83139_tbx-v-f-norm-ntv-cpms.csv.gz
INSDC Project Accessions:GEO Series Accessions:

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Analysis Portals

None

Project Label

HumanEndocrinePancreas

Species

Homo sapiens

Sample Type

specimens

Anatomical Entity

pancreas

Organ Part

islet of Langerhans

Selected Cell Types

pancreatic endocrine cell

Disease Status (Specimen)

3 disease statuses

Disease Status (Donor)

3 disease statuses

Development Stage

2 development stages

Library Construction Method

Smart-seq2

Nucleic Acid Source

single cell

Paired End

false

Analysis Protocol

MultiSampleSmartSeq2_v2.2.6, SmartSeq2SingleSample_v5.1.5

File Format

5 file formats

Cell Count Estimate

635

Donor Count

8
bai1,189 file(s)bam1,189 file(s)csv1 file(s)fastq.gz1,189 file(s)loom1 file(s)