HCA Data Explorer

Single Cell RNA Sequencing of Human Milk-Derived Cells Reveals Sub-Populations of Mammary Epithelial Cells with Molecular Signatures of Progenitor and Mature States: a Novel, Non-invasive Framework for Investigating Human Lactation Physiology

Updated January 23, 2023

Milk derived live mammary epithelial cells were characterized by single cell RNA sequencing. Overall design: Cells were pelleted from mid-feed milk samples prior to cryopreservation and subsequent analysis by single cell RNA sequencing.

Michael C RudolphUniversity of Colorado Anschutz Medical Campusmichael-rudolph@ouhsc.edu
Michael C Rudolph (Principal Investigator)1
1University of Colorado Anschutz Medical Campus
Rachel Schwartz

To reference this project, please use the following link:

https://explore.data.humancellatlas.org/projects/8999b456-6fa6-438b-ab17-b62b1d8ec0c3
None
INSDC Project Accessions:GEO Series Accessions:INSDC Study Accessions:

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Analysis Portals

None

Project Label

MilkEpithelialCells

Species

Homo sapiens

Sample Type

specimens

Anatomical Entity

breast

Organ Part

breast epithelium

Selected Cell Types

mammary gland epithelial cell

Disease Status (Specimen)

normal

Disease Status (Donor)

gestational diabetes

Development Stage

human adult stage

Library Construction Method

10x 3' v3

Nucleic Acid Source

single cell

Paired End

false

Analysis Protocol

analysis_protocol_1

File Format

4 file formats

Cell Count Estimate

3.7k

Donor Count

2
fastq.gz32 file(s)mtx.gz1 file(s)tsv.gz2 file(s)xlsx1 file(s)