HCA Data Explorer

A single cell atlas of human cornea that defines its development, limbal progenitor cells and their interactions with the immune cells.

Updated July 19, 2022

To study the development and composition of human ocular surface, the authors performed single cell (sc) RNA-Seq at key embryonic, fetal and adult stages and generated the first atlas of the corneal and conjunctival cell types from development to adulthood.

Lyle ArmstrongNewcastle Universitylyle.armstrong@ncl.ac.uk
Majlinda LakoNewcastle Universitymajlinda.lako@newcastle.ac.uk
Joseph Collin1
Rachel Queen1
Darin Zerti1
Sanja Bojic1
Birthe Dorgau1
Nicky Moyse2
Marina Moya Molina1
Chunbo Yang1
Sunanda Dey3
Gary Reynolds1
Rafiqul Hussain1
Jonathan M Coxhead1
Steven Lisgo1
Deborah Henderson1
Agatha Joseph4
Paul Rooney4
Saurabh Ghosh5
Lucy Clarke6
Che Connon1
Muzlifah Haniffa1
Francisco Figueiredo1
Lyle Armstrong1
Majlinda Lako1
1Newcastle University
2Newcastle University and Newcastle Upon Tyne Hospitals NHS Foundation Trust
3The Ruth and Bruce Rappaport Faculty of Medicine, Technion - Israel Institute of Technology
4NHS Blood and Transplant Tissue and Eye Services
5Sunderland Eye Infirmary, South Tyneside and Sunderland NHS Foundation Trust
6Royal Victoria Infirmary and Newcastle University
Ami Day

To reference this project, please use the following link:

https://explore.data.humancellatlas.org/projects/6ac8e777-f9a0-4288-b5b0-446e8eba3078

Supplementary links are provided by contributors and represent items such as additional data which can’t be hosted here; code that was used to analyze this data; or tools and visualizations associated with this specific dataset.

1.http://retinalstemcellresearch.co.uk/CorneaCellAtlas/
INSDC Project Accessions:GEO Series Accessions:INSDC Study Accessions:

Downloaded and exported data is governed by the HCA Data Release Policy and licensed under the Creative Commons Attribution 4.0 International License (CC BY 4.0). For more information please see our Data Use Agreement.

Analysis Portals

UCSC Cell BrowserUCSC Cell Browser

Project Label

HumanCorneaDevelopment

Species

Homo sapiens

Sample Type

2 sample types

Anatomical Entity

eye

Organ Part

2 organ parts

Selected Cell Types

Unspecified

Model Organ

eye

Disease Status (Specimen)

3 disease statuses

Disease Status (Donor)

3 disease statuses

Development Stage

10 development stages

Library Construction Method

2 library construction methods

Nucleic Acid Source

2 nucleic acid sources

Paired End

true

Analysis Protocol

analysis_protocol_cell_type_annotation, analysis_protocol_scATACseq, analysis_protocol_scRNAseq

File Format

4 file formats

Cell Count Estimate

21.3k

Donor Count

25
csv.gz1 file(s)fastq.gz100 file(s)tsv10 file(s)txt.gz8 file(s)