HCA Data Explorer

Re-evaluation of human BDCA-2+ DC during acute sterile skin inflammation.

Updated September 25, 2023

Transcriptomic profiling of skin wounds in a time-course manner. Full thickness punch biopsies were performed on the flank of healthy donors to create skin wounds. This was followed by larger biopsies, to encompass the original wounds, taken between one to four days later. The skin biopsies and blood samples were subjected to single-cell RNA sequencing analysis.

Graham OggOxford National Institute for Health Research Biomedical Research Centre, Medical Research Council Weatherall Institute of Molecular Medicine, University of Oxfordgraham.ogg@ndm.ox.ac.uk
Yi-Ling Chen1
Tomas Gomes2
Clare S Hardman1
Felipe A Vieira Braga2
Danuta Gutowska-Owsiak1
Maryam Salimi1
Nicki Gray3
David A Duncan4
Gary Reynolds5
David Johnson6
Mariolina Salio1
Vincenzo Cerundolo1
Jillian L Barlow7
Andrew N J McKenzie7
Sarah A Teichmann2
Muzlifah Haniffa2
Graham Ogg1
1Oxford National Institute for Health Research Biomedical Research Centre, Medical Research Council Weatherall Institute of Molecular Medicine, University of Oxford
2Wellcome Sanger Institute
3Weatherall Institute of Molecular Medicine
4Diamond Light Source, Harwell Science and Innovation Campus
5Institute of Cellular Medicine
6John Radcliffe Hospital, Oxford University Hospitals National Health Services Foundation Trust
7Medical Research Council Laboratory of Molecular Biology
Ami Day

To reference this project, please use the following link:

https://explore.data.humancellatlas.org/projects/67a3de09-45b9-49c3-a068-ff4665daa50e
None
INSDC Project Accessions:
ERP118283
GEO Series Accessions:
GSE139834, GSE139952
Array Express Accessions:
E-MTAB-8498
INSDC Study Accessions:
PRJEB35252

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Analysis Portals

None

Project Label

AcuteSkinInflammation

Species

Homo sapiens

Sample Type

specimens

Anatomical Entity

2 anatomical entities

Organ Part

Unspecified

Selected Cell Types

5 cell types

Disease Status (Specimen)

normal

Disease Status (Donor)

normal

Development Stage

human adult stage

Library Construction Method

Smart-seq2

Nucleic Acid Source

single cell

Paired End

true

Analysis Protocol

MultiSampleSmartSeq2_v2.2.6, SmartSeq2SingleSample_v5.1.5

File Format

5 file formats

Cell Count Estimate

455

Donor Count

1
bai719 file(s)bam719 file(s)fastq.gz1,464 file(s)loom2 file(s)xlsx1 file(s)