HCA Data Explorer

Differentiation of Human Intestinal Organoids with Endogenous Vascular Endothelial Cells

Updated November 10, 2021

Human pluripotent stem cell (hPSC)-derived intestinal organoids (HIOs) lack some cellular populations found in the native organ, including vasculature. Using single-cell RNA sequencing (scRNA-seq), we have identified a population of endothelial cells (ECs) present early in HIO differentiation that declines over time in culture. Here, we developed a method to expand and maintain this endogenous population of ECs within HIOs (vHIOs). Given that ECs possess organ-specific gene expression, morphology, and function, we used bulk RNA-seq and scRNA-seq to interrogate the developing human intestine, lung, and kidney in order to identify organ-enriched EC gene signatures. By comparing these gene signatures and validated markers to HIO ECs, we find that HIO ECs grown in vitro share the highest similarity with native intestinal ECs relative to kidney and lung. Together, these data demonstrate that HIOs can co-differentiate a native EC population that is properly patterned with an intestine-specific EC transcriptional signature in vitro.

Jason SpenceUniversity of Michiganspencejr@med.umich.edu

Differentiation of Human Intestinal Organoids with Endogenous Vascular Endothelial Cells

Emily M. Holloway1
Joshua H. Wu1
Michael Czerwinski1
Caden W. Sweet1
Angeline Wu1
Yu-Hwai Tsai1
Sha Huang1
Amy E. Stoddard1
Meghan M. Capeling1
Ian A. Glass2
Jason Spence1
1University of Michigan
2University of Washington
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To reference this project, please use the following link:

https://explore.data.humancellatlas.org/projects/5eafb94b-02d8-423e-81b8-3673da319ca0

Supplementary links are provided by contributors and represent items such as additional data which can’t be hosted here; code that was used to analyze this data; or tools and visualizations associated with this specific dataset.

1.http://www.jasonspencelab.com/protocols
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Analysis Portals

None

Project Label

DifferentiationofHumanIntestinalOrganoidswithEndog

Species

Homo sapiens

Sample Type

2 sample types

Anatomical Entity

3 anatomical entities

Organ Part

4 organ parts

Selected Cell Types

Unspecified

Model Organ

intestine

Disease Status (Specimen)

Unspecified

Disease Status (Donor)

Unspecified

Development Stage

6 development stages

Library Construction Method

2 library construction methods

Nucleic Acid Source

single cell

Paired End

false

File Format

fastq

Cell Count Estimate

Unspecified

Donor Count

7
fastq109 file(s)