HCA Data Explorer

Single-cell transcriptomes of the aging human skin reveal loss of fibroblast priming

Updated August 3, 2023

Fibroblasts are the main dermal cell type and are essential for the architecture and function of human skin. Important differences have been described between fibroblasts localized in distinct dermal layers, and these cells are also known to perform varied functions. However, this phenomenon has not been analyzed comprehensively yet. Here we have used single-cell RNA sequencing to analyze >15,000 cells from a sun-protected area in young and old donors. Our results define four main fibroblast subpopulations that can be spatially localized and functionally distinguished. Importantly, intrinsic aging reduces this fibroblast ‘priming’, generates distinct expression patterns of skin aging-associated genes, and substantially reduces the interactions of dermal fibroblasts with other skin cell types. Our work thus provides comprehensive evidence for a functional specialization of human dermal fibroblasts and suggests that the age-related loss of fibroblast priming contributes to human skin aging. Overall design: Overall 5 samples were analyzed. Two of them are from young subjects (age=25) and serve as replicates, three of them are from old subjects (age >= 50) and serve as replicates.

Manuel Rodríguez-ParedesGerman Cancer Research Centerm.rodriguez@dkfz.de
Frank LykoGerman Cancer Research Centerf.lyko@dkfz.de
Llorenç Solé-Boldo (Experimental Scientist)1
Günter Raddatz (Experimental Scientist)1
Sabrina Schütz (Experimental Scientist)1
Jan-Philipp Mallm (Experimental Scientist)1
Karsten Rippe (Experimental Scientist)1
Anke S Lonsdorf (Experimental Scientist)2
Manuel Rodríguez-Paredes (Principal Investigator)1
Frank Lyko (Principal Investigator)1
1German Cancer Research Center
2Ruprecht-Karls University of Heidelberg Hospital
Marion F Shadbolt

To reference this project, please use the following link:

https://explore.data.humancellatlas.org/projects/51f02950-ee25-4f4b-8d07-59aa99bb3498
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Analysis Portals

CZ CELLxGENECZ CELLxGENE
Genome BrowserGenome Browser
UCSC Cell BrowserUCSC Cell Browser

Project Label

scAgingHumanMaleSkin

Species

Homo sapiens

Sample Type

specimens

Anatomical Entity

skin of body

Organ Part

skin of pelvis

Selected Cell Types

Unspecified

Disease Status (Specimen)

normal

Disease Status (Donor)

normal

Development Stage

adult

Library Construction Method

10x 3' v2

Nucleic Acid Source

single cell

Paired End

false

Analysis Protocol

analysis_protocol

File Format

4 file formats

Cell Count Estimate

15.5k

Donor Count

5
fastq.gz10 file(s)mtx.gz2 file(s)tsv.gz4 file(s)xlsx1 file(s)