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Single-cell RNA sequencing of human nasal swab from healthy donors

Updated January 23, 2023

Understanding on pathogenesis of COVID-19 is rapidly growing, but primary target cells of SARS-CoV-2 infection is still not known. Here, we performed single cell RNA sequencing on human nasal swab from healthy donors to investigate the expression patterns of host cell entry factors of SARS-CoV-2. Overall design: Single cells from healthy donors' nasal swabs were isolated and single-cell transcriptome libraries were generated

None
Ji Hoon Ahn1
JungMo Kim1
Seon Pyo Hong1
Sung Yong Choi2
Myung Jin Yang3
Young Seok Ju3
Young Tae Kim4
Ho Min Kim3
M D Tazikur Rahman5
Man Ki Chung6
Sang Duk Hong6
Hosung Bae1
Chang-Seop Lee7
Gou Young Koh1
1Center for Vascular Research, Institute for Basic Science (IBS), Daejeon, Korea, Republic of.
2Department of Otorhinolaryngology-Head and Neck Surgery, Uijeongbu Eulji Medical Center, Uijeongbu, Korea, Republic of.
3Graduate School of Medical Science and Engineering, Korea Advanced Institute of Science and Technology (KAIST), Daejeon, Korea, Republic of.
4Department of Thoracic and Cardiovascular Surgery, Seoul National University Hospital, Seoul, Korea, Republic of.
5Department of Medical Science, Jeonbuk National University Medical School, Jeonju, Korea, Republic of.
6Department of Otorhinolaryngology-Head and Neck Surgery, Samsung Medical Center, Seoul, Korea, Republic of.
7Department of Internal Medicine, Jeonbuk National University Medical School, Jeonju, Korea, Republic of.
Tiana Pereira

To reference this project, please use the following link:

https://explore.data.humancellatlas.org/projects/414acced-eba0-440f-b721-befbc5642bef
None
INSDC Project Accessions:
SRP313527
GEO Series Accessions:
GSE171488
INSDC Study Accessions:
PRJNA719801

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Analysis Portals

None

Project Label

scRNACovidNasalSwab

Species

Homo sapiens

Sample Type

specimens

Anatomical Entity

nose

Organ Part

nasal cavity epithelium

Selected Cell Types

nasal cavity respiratory epithelium epithelial cell of viscerocranial mucosa

Disease Status (Specimen)

normal

Disease Status (Donor)

normal

Development Stage

human adult stage

Library Construction Method

barcoded plate-based single cell RNA-seq

Nucleic Acid Source

single cell

Paired End

true

Analysis Protocol

analysis_protocol_1

File Format

3 file formats

Cell Count Estimate

Unspecified

Donor Count

2
fastq.gz4 file(s)txt.gz2 file(s)xlsx1 file(s)