HCA Data Explorer

Single-Cell RNAseq analysis of diffuse neoplastic infiltrating cells at the migrating front of human glioblastoma

Updated January 19, 2022

We used single-cell RNAseq to investigate the heterogeneity of glioblastoma tumors and assess differential expression between cells within and in proximity of the tumor.

Spyros DarmanisStanford Universityspyros.darmanis@gmail.com
Spyros Darmanis (Experimental Scientist)1
Steven A Sloan (Experimental Scientist)1
1Stanford University
Karina Hernandez
Tiana Pereira
Parisa Nejad

To reference this project, please use the following link:

https://explore.data.humancellatlas.org/projects/2d846095-8a33-4f3c-97d4-585bafac13b4

Supplementary links are provided by contributors and represent items such as additional data which can’t be hosted here; code that was used to analyze this data; or tools and visualizations associated with this specific dataset.

1.ftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE84nnn/GSE84465/suppl/GSE84465_GBM_All_data.csv.gz,ftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE84nnn/GSE84465/suppl/GSE84465_RAW.tar
INSDC Project Accessions:GEO Series Accessions:

Downloaded and exported data is governed by the HCA Data Release Policy and licensed under the Creative Commons Attribution 4.0 International License (CC BY 4.0). For more information please see our Data Use Agreement.

Analysis Portals

CZ CELLxGENECZ CELLxGENE
UCSC Cell BrowserUCSC Cell Browser

Project Label

InfiltratingNeoplasticCellsHumanGlioblastoma

Species

Homo sapiens

Sample Type

specimens

Anatomical Entity

brain

Organ Part

cerebral cortex

Selected Cell Types

7 cell types

Disease Status (Specimen)

glioblastoma (disease)

Disease Status (Donor)

glioblastoma (disease)

Development Stage

human adult stage

Library Construction Method

Smart-seq2

Nucleic Acid Source

single cell

Paired End

true

Analysis Protocol

MultiSampleSmartSeq2_v2.2.6, SmartSeq2SingleSample_v5.1.5

File Format

5 file formats

Cell Count Estimate

3.6k

Donor Count

4
bai3,588 file(s)bam3,588 file(s)csv4 file(s)fastq.gz7,176 file(s)loom1 file(s)