HCA Data Explorer

Single cell RNA-seq of HeLa CCL2

Updated August 30, 2022

We measured 720 HeLa-CCL2 cells (288 from the 9th passage, 288 from the 14th passage and 144 from the 20th passage) in this work on an Illumina HiSeq 4000 platform (sequenced by Novogene, China) for 150 bp paired-end sequencing. The protocol was based on Tang’s modification on SMART-Seq2. Single cells were captured by mouth pipette under the stereomicroscope(Nikon SZM745)and followed by the protocol. Every cell was assigned with 250 M raw data on average.

Xiao-Dong SuPeking Universityxdusu@pku.edu.cn
Xiao-Dong Su (Experimental Scientist)1
1Peking University
Rachel Schwartz

To reference this project, please use the following link:

https://explore.data.humancellatlas.org/projects/18d4aae2-8363-4e00-8eeb-b9e568402cf8
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Analysis Portals

None

Project Label

scRNAofHelaCCL2

Species

Homo sapiens

Sample Type

cellLines

Anatomical Entity

uterus

Organ Part

cervix

Selected Cell Types

epithelial cell of cervix

Model Organ

uterus

Disease Status (Specimen)

adenocarcinoma

Disease Status (Donor)

adenocarcinoma

Development Stage

human adult stage

Library Construction Method

Smart-like

Nucleic Acid Source

single cell

Paired End

true

Analysis Protocol

analysis_protocol_1

File Format

3 file formats

Cell Count Estimate

720

Donor Count

1
fastq.gz16 file(s)tar1 file(s)xlsx1 file(s)