Single cell profiling of human induced dendritic cells generated by direct reprogramming of embryonic fibroblasts
Updated January 31, 2024Dendritic cells (DCs) are professional antigen-presenting cells specialized in the recognition, processing and presentation of antigens to T-cells, inducing adaptive immune responses. We have identified PU.1, IRF8 and BATF3 transcription factors (TFs) to reprogram human fibroblasts into DC type 1 (DC1). Induced DCs acquire a step-wise DC1 transcriptional signature at the single cell level as assessed with droplet based scRNA-seq. Hence, we provide evidence that antigen presentation can be dynamically programmed by a small combination of TFs in human cells. Moreover, it represents a platform for generating autologous DC1, the most attractive DC subset for cancer immunotherapy.
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Analysis Portals
NoneProject Label
Reprogrammed_Dendritic_CellsSpecies
Sample Type
Anatomical Entity
Organ Part
Selected Cell Types
Model Organ
Disease Status (Specimen)
Disease Status (Donor)
Development Stage
Library Construction Method
Nucleic Acid Source
Paired End
falseAnalysis Protocol
optimus_post_processing_v1.0.0, optimus_v4.2.2File Format
Cell Count Estimate
6.3kDonor Count
1