HCA Data Explorer

The Tabula Sapiens: a single cell transcriptomic atlas of multiple organs from individual human donors

Updated August 30, 2022

In recent years there has been tremendous progress towards deep molecular characterization of cell types using single cell transcriptome sequencing. Here we report a single cell transcriptomic atlas comprising nearly 500,000 cells from 24 different human tissues and organs. In several instances multiple organs were analyzed from the same donor. Analyzing organs from the same individual controls for genetic background, age, environment, and epigenetic effects, and enables a detailed comparison of cell types that are shared between tissues. This resource provides a rich molecular characterization of more than 400 cell types, their distribution across tissues, and detailed information about tissue specific variation in gene expression. We have used the fact that multiple tissues came from the same donor to study the clonal distribution of T cells between tissues, to understand the tissue specific mutation rate in B cells, and to analyze the cell cycle state and proliferative potential of shared cell types across tissues. Finally, we have also used this data to characterize cell type specific RNA splicing and how such splicing varies across tissues within an individual.

Stephen R QuakeCZ Biohubsteve@czbiohub.org
Stephen R Quake (Principal Investigator)1
1CZ Biohub
Parisa Nejad
Brittney D Wick
Rachel Schwartz

To reference this project, please use the following link:

https://explore.data.humancellatlas.org/projects/10201832-7c73-4033-9b65-3ef13d81656a

Supplementary links are provided by contributors and represent items such as additional data which can’t be hosted here; code that was used to analyze this data; or tools and visualizations associated with this specific dataset.

1.https://tabula-sapiens-portal.ds.czbiohub.org/home
None

For information regarding data sharing and data use, please see our Data Access Policy.

Analysis Portals

CZ CELLxGENECZ CELLxGENE
Genome BrowserGenome Browser
UCSC Cell BrowserUCSC Cell Browser

Project Label

tabulaSapiens

Species

Homo sapiens

Sample Type

specimens

Anatomical Entity

24 anatomical entities

Organ Part

Unspecified

Selected Cell Types

462 cell types

Disease Status (Specimen)

normal

Disease Status (Donor)

11 disease statuses

Development Stage

human adult stage

Library Construction Method

3 library construction methods

Nucleic Acid Source

single cell

Paired End

false, true

Analysis Protocol

analysis_protocol_1, analysis_protocol_2

File Format

2 file formats

Cell Count Estimate

481.1k

Donor Count

15
h5ad.zip1 file(s)xlsx1 file(s)