HCA Data Explorer

Single-cell RNA-Seq of human hepatocyte-derived liver progenitor-like cells

Updated July 19, 2022

The authors describe a protocol for efficient conversion of human primary hepatocytes into liver progenitor-like cells (HepLPCs) through delivery of developmentally relevant cues. 10X Genomics Single Cell Gene Expression was performed to comprehensively characterize cell subpopulations within the human hepatocyte-derived liver progenitor-like cells (HepLPCs). Human hepatocyte-derived liver progenitor-like cells (HepLPCs) were derived from one donor and cultured in transition and expansion medium until passage 5.

Bo ZhaiDepartment of Interventional Oncology, Renji Hospital, Jiaotong University School of Medicinezhaiboshi@sina.com
Zhi-Ying HeInstitute for Regenerative Medicine, Shanghai East Hospital, School of Life Sciences and Technology, Tongji Universityzhiyinghe2002@163.com
Hong-Yang WangInternational Cooperation Laboratory on Signal Transduction, Eastern Hepatobiliary Surgery Hospital, Second Military Medical Universityhywangk@vip.sina.com
He-Xin YanInternational Cooperation Laboratory on Signal Transduction, Eastern Hepatobiliary Surgery Hospital, Second Military Medical Universityhexinyw@163.com
Gong-Bo Fu1
Wei-Jian Huang1
Min Zeng1
Xu Zhou1
Hong-Ping Wu2
Chang-Cheng Liu3
Han Wu1
Jun Weng4
Hong-Dan Zhang5
Yong-Chao Cai6
Charles Ashton7
Min Ding8
Dan Tang9
Bao-Hua Zhang2
Yi Gao4
Wei-Feng Yu9
Bo Zhai8
Zhi-Ying He3
Hong-Yang Wang1
He-Xin Yan1
1International Cooperation Laboratory on Signal Transduction, Eastern Hepatobiliary Surgery Hospital, Second Military Medical University
2National Center for Liver Cancer
3Institute for Regenerative Medicine, Shanghai East Hospital, School of Life Sciences and Technology, Tongji University
4Department of Hepatobiliary Surgery II, Zhujiang Hospital, Southern Medical University
5Celliver Biotechnology Inc
6Institute for Regenerative Medicine, Shanghai East Hospital, School of Life Sciences and Technology, Tongji University,
7Zilkha Neurogenetic Institute, University of Southern California
8Department of Interventional Oncology, Renji Hospital, Jiaotong University School of Medicine
9Department of Anesthesiology and Critical Care Medicine, Renji Hospital, Jiaotong University School of Medicine
Ami Day

To reference this project, please use the following link:

https://explore.data.humancellatlas.org/projects/074a9f88-729a-455d-bca5-0ce80edf0cea
None
INSDC Project Accessions:GEO Series Accessions:INSDC Study Accessions:

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Analysis Portals

None

Project Label

HumanHepatocyteDifferentiation

Species

Homo sapiens

Sample Type

specimens

Anatomical Entity

liver

Organ Part

Unspecified

Selected Cell Types

Unspecified

Disease Status (Specimen)

normal

Disease Status (Donor)

liver hemangioma

Development Stage

human adult stage

Library Construction Method

10x 3' v2

Nucleic Acid Source

single cell

Paired End

false

Analysis Protocol

analysis_protocol

File Format

2 file formats

Cell Count Estimate

7.5k

Donor Count

1
fastq.gz12 file(s)tar1 file(s)