HCA Data Explorer

Single-cell transcriptome analysis reveals the dynamics of human immune cells during early fetal skin development

Updated January 20, 2022

The immune system of skin develops in stages in mice. However, the developmental dynamics of immune cells in human skin remains elusive. Here we perform transcriptome profiling of CD45+ hematopoietic cells in human fetal skin at an estimated gestational age of 10–17 weeks by single-cell RNA sequencing. A total of 13 immune cell types are identified. Skin macrophages show heterogeneity and dynamic over the course of skin development. A major shift in lymphoid cell developmental states occurs from the first to the second trimester that implied an in situ differentiation process. Gene expression analysis reveals a typical developmental program in immune cells in accordance with their functional maturation, possibly involving metabolic reprogramming. Finally, we identify transcription factors (TFs) that potentially regulate cellular transitions by comparing TFs and TF-target gene networks. These findings provide detailed insight into how the immune system of the human skin is established during development Overall design: Here we perform transcriptome profiling of CD45+ hematopoietic cells in human fetal skin at an estimated gestational age of 10–17 weeks by single-cell RNA sequencing

Yingping XuSouthern Medical Universityyingpxu@126.com
Yingping Xu (Experimental Scientist)1
Jun Zhang1
Yongfei Hu1
Xuefei Li1
Lihua Sun1
Yu Peng1
Yuzhe Sun1
Bing Liu2
Zhilei Bian3
Zhili Rong1
1Southern Medical University
2Fifth Medical Center of Chinese PLA General Hospital
3Zhengzhou University
Parisa Nejad

To reference this project, please use the following link:

https://explore.data.humancellatlas.org/projects/06c7dd8d-6cc6-4b79-b795-8805c47d36e1
None
INSDC Project Accessions:GEO Series Accessions:INSDC Study Accessions:

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Analysis Portals

None

Project Label

immuneCellsDuringSkinDevelopment

Species

Homo sapiens

Sample Type

specimens

Anatomical Entity

skin of body

Organ Part

Unspecified

Selected Cell Types

Unspecified

Disease Status (Specimen)

normal

Disease Status (Donor)

normal

Development Stage

fetal stage

Library Construction Method

2 library construction methods

Nucleic Acid Source

single cell

Paired End

false

Analysis Protocol

analysis_protocol_1

File Format

3 file formats

Cell Count Estimate

57.4k

Donor Count

6
fastq.gz12 file(s)mtx.gz6 file(s)tsv.gz12 file(s)