HCA Data Explorer

Single cell RNA sequencing of human failing heart

Updated September 15, 2022

we performed single cell RNA sequencing (scRNA-seq) in both normal and failed human heart. CD45- cells in the myocardium and immune cells (CD45+ cells) were isolated from myocardium and corresponding peripheral blood were sorted for scRNA-seq. Our findings were also validated with bulk RNAseq. Overall design: Establishing a comprehensive cell atlas of immune cells in human heart failure at single cell level.

Shengshou HuChinese Academy of Medical Sciencetccbrm@gmail.com
Shengshou Hu (Experimental Scientist)1
1Chinese Academy of Medical Science
Parisa Nejad

To reference this project, please use the following link:

https://explore.data.humancellatlas.org/projects/065e6c13-ad6b-46a3-8075-c3137eb03068
None
INSDC Project Accessions:GEO Series Accessions:INSDC Study Accessions:

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Analysis Portals

None

Project Label

scRNAseqOfFailingHumanHeart

Species

Homo sapiens

Sample Type

specimens

Anatomical Entity

2 anatomical entities

Organ Part

2 organ parts

Selected Cell Types

Unspecified

Disease Status (Specimen)

3 disease statuses

Disease Status (Donor)

3 disease statuses

Development Stage

human adult stage

Library Construction Method

10x 3' transcription profiling

Nucleic Acid Source

single cell

Paired End

false

Analysis Protocol

analysis_protocol_1

File Format

4 file formats

Cell Count Estimate

200.6k

Donor Count

6
fastq.gz59 file(s)mtx.gz30 file(s)tsv.gz60 file(s)xlsx1 file(s)