HCA Data Explorer

Single-cell RNA-seq reveals heterogeneity within human pre-cDCs

Updated January 19, 2022

Here, we use a microfluidics-based approach to prepare single-cell RNA-Seq libraries from 164 primary human conventional dendritic cells (cDCs) as well as cord blood (452) and blood (341) pre-cDCs. We examined heterogeneity between individual cells to document potential subpopulations within human cDCs and pre-cDCs.

Thiago Y OliveiraThe Rockefeller Universitytoliveira@rockefeller.edu
Thiago Y Oliveira (Experimental Scientist)1
Michel C Nussenzweig (Principal Investigator)1
1The Rockefeller University
Parisa Nejad
Kamron Mojabe
Beagan Nguy

To reference this project, please use the following link:

https://explore.data.humancellatlas.org/projects/05be4f37-4506-429b-b112-506444507d62

Supplementary links are provided by contributors and represent items such as additional data which can’t be hosted here; code that was used to analyze this data; or tools and visualizations associated with this specific dataset.

1.ftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE89nnn/GSE89232/suppl/GSE89232_expMatrix.txt.gz
INSDC Project Accessions:GEO Series Accessions:

Downloaded and exported data is governed by the HCA Data Release Policy and licensed under the Creative Commons Attribution 4.0 International License (CC BY 4.0). For more information please see our Data Use Agreement.

Analysis Portals

None

Project Label

HumanDCsFromPre-cDCs

Species

Homo sapiens

Sample Type

specimens

Anatomical Entity

blood

Organ Part

2 organ parts

Selected Cell Types

2 cell types

Disease Status (Specimen)

Unspecified

Disease Status (Donor)

normal

Development Stage

human adult stage

Library Construction Method

Smart-seq2

Nucleic Acid Source

single cell

Paired End

false

Analysis Protocol

MultiSampleSmartSeq2_v2.2.6, SmartSeq2SingleSample_v5.1.5

File Format

5 file formats

Cell Count Estimate

957

Donor Count

2
bai1,543 file(s)bam1,543 file(s)fastq.gz1,543 file(s)loom1 file(s)txt.gz1 file(s)